畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (3): 477-482.doi: 10.11843/j.issn.0366-6964.2014.03.019

• 基础兽医 • 上一篇    下一篇

鸡鼻相关淋巴组织中免疫细胞的定位分布

杨树宝1,张英楠2,3,马馨1,杨丽华2,姚纪元1,钱爱东1,栾维民1,4*   

  1. (1.吉林农业大学动物科技学院,长春 130118;2.长春科技学院,长春 130600;3.吉林农业大学生命科学学院,长春 130118; 4.吉林农业大学动物生产及产品质量安全教育部重点实验室,长春 130118)
  • 收稿日期:2013-09-04 出版日期:2014-03-23 发布日期:2014-03-23
  • 通讯作者: 栾维民,E-mail:luanweimin1957@163.com
  • 作者简介:杨树宝(1981-),男,吉林临江人,讲师,博士生,主要从事动物免疫学研究,E-mail: yangshubao1981@163.com
  • 基金资助:

    国家自然科学基金项目(30901065;31372391); 吉林省科技发展计划项目(201205059)

Localization of Immune Cells in Chicken Nasal-associate Lymphoid Tissues

YANG Shu-bao1,ZHANG Ying-nan2,3,MA Xin1,YANG Li-hua2,YAO Ji-yuan1,QIAN Ai-dong1 ,LUAN Wei-min1,4*   

  1. (1.College of Animal Science and technology,Jilin Agricultural University,Changchun 130118, China;2.Changchun University of Science and Technology,Changchun 130600, China; 3.College of Life Science,Jilin Agricultural University,Changchun 130118, China; 4.Key Laboratory of Animal Production & Product Quality and Security of Ministry of Education,Jilin Agricultural University,Changchun 130118, China)
  • Received:2013-09-04 Online:2014-03-23 Published:2014-03-23

摘要:

为了解鸡鼻相关淋巴组织(NALT)的免疫状态,选择21日龄海兰白鸡的鼻组织,利用免疫组织化学方法研究不同免疫细胞的定位分布及相对数量。结果显示,鸡NALT主要定位于鼻泪管、鼻后孔和鼻甲黏膜中,其中以鼻泪管黏膜中的NALT最为发达。CD3+和CD4+T淋巴细胞聚集在鼻黏膜上皮下固有层底端,占据NALT的中下部区域,CD8+细胞数量较少,分散在NALT的中部;Bu-1+细胞为NALT中的主要细胞群,并密集而范围较大地分布在NALT外周,以绝对优势占据NALT的中上部区域;抗体生成细胞以IgA+和IgM+细胞为主,主要散布于NALT中央;Ma+细胞主要分布在NALT的深层疏松结缔组织中;MHC-Ⅱ+细胞则密集地分布于整个NALT中,为NALT中数量最多的免疫细胞。结果表明,鸡NALT中存在多种免疫细胞,在上呼吸道免疫防御方面起重要作用。

Abstract:

The aim of this study was to explore the immune state of nasal-associated lymphoid tissue(NALT)in chicken.Nasal tissues were taken from 21-day-old Hailan White chicks to investigate the localization and the relative quantities of different immune cells by immunohistochemistry method.The results showed that NALT in chicken was mainly located in the mucosa of nasolacrimal duct,posterior nasal apertures and conchae nasals.NALT in the mucosa of nasolacrimal duct was the most developed.CD3+ and CD4+ T lymphocytes mainly distributed at the bottom of lamina propria under epithelium in NALT,which were found predominantly at the middle and inferior part of lamina propria.The number of CD8+ lymphocytes was low,and they scattered in middle part of NALT.Bu-1+ lymphocytes constituted the main population in NALT,and they distributed around the peripheral NALT densely and widely.Bu-1+ cells occupied the middle and inferior part overwhelmingly.The main antibody producing cells were IgA+ or IgM+ cells,and they scattered in the middle part of NALT predominantly.Ma+ cells mainly distributed in the deep loose connective tissue.MHC-Ⅱ+ cells distributed densely throughout the NALT.The number of MHC-Ⅱ+ cells was highest among the immune cells in NALT.The results demonstrated that there were several kinds of immune cells in NALT of chicken.These immune cells played an important role in immune defence at the upper respiratory tract.

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